Background: Due to its extensive arsenal of virulence factors and inherent resistance\nto antibiotics, Pseudomonas aeruginosa is a threat particularly in immunocompromised patients.\nConsidering the central role of quorum sensing in the production of virulence factors, inhibition\nof bacterial communication mechanism constitute an opportunity to attenuate pathogenicity of\nbacteria resistant to available antibiotics. Our study aimed to assess the anti-quorum sensing\nactivity of Anogeissus leiocarpus, traditionally used in Burkina Faso, for the treatment of infected\nburn wounds. Methods: Investigations were carried out on methanol extract from A. leiocarpus\nstem bark. The reporter strains Chromobacterium violaceum CV026 and P. aeruginosa PAO1 derivatives\nwere used to evidence any interference with the bacterial quorum sensing and expression of related\ngenes. P. aeruginosa PAO1 was used to measure the impact on pyocyanin production. Results: At\na sub-inhibitory concentration (100 �¼g/mL), A. leiocarpus methanol extract quenched the quorum\nsensing mechanism of P. aeruginosa PAO1 by down-streaming the rhlR gene, with a subsequent\nreduction of pyocyanin production. Moreover, the antioxidant polyphenols evidenced are able to\nreduce the oxidative stress induced by pyocyanin. Conclusion: The antioxidant and anti-quorum\nsensing activities of A. leiocarpus stem bark could justify its traditional use in the treatment of infected\nburn wounds.
Loading....